Adherent cells ks y 1 m21 hey t1 were harvested on the following day using a rubber policeman.
Rubber policeman sigma.
Hydrochloric acid hcl sodium hydroxide naoh ethylenediaminetetraacetic acid edta dithiothreitol dtt sodium chloride.
Search results for rubber policemen at sigma aldrich.
The versatile double ended styles have two different shaped blades for precise tailoring to specific testing applications.
The cells were scraped with a sterile rubber policeman sigma aldrich collected in pbs in 1 5 ml tubes eppendorf and stored at 80 c.
Qiashredder spin columns qiagen 3.
Please select more than one item to compare.
Please select more than one item to compare.
Select up to 4 products.
Blunt tapered or round spatula tips in any size are designed to not scratch or damage surface when handling.
Phosphate buffered saline ph 7 2 with mg ca pbs sigma magnesium chloride mgcl 2 calcium chloride cacl 2 sodium dodecyl sulfate sds tris base.
Select up to 4 products.
Please select more than one item to compare.
The instruments feature a flat.
Ideal for scraping and mixing transfer collect or stir biological samples with complete ease by using the multi purpose spatulas.
Natural rubber scrapers rod diam.
Cells were grown to 70 confluency in 150 mm dishes after which tgf β1 was added at a final concentration of 100 pm.
A policeman is a hand held flexible natural rubber or plastic scraper.
A policeman also comes in various sizes shapes and types.
Each cell pellet was lysed in 112 5 μl of lysis buffer 10 mm tris ph 8 0 10 mm edta ph 8 0 1 np 40 1 mm pmsf for 20 min at 4 c diluted with 634 5 μl of dilution buffer 10 mm tris ph 8 0 and.
Search results for dichloromethane at sigma aldrich.
Select up to 4 products.
After 24 hrs cells were washed 3 times with ice cold tris buffered saline tbs collected in tbs using a rubber policeman and pelleted by centrifugation 1500 rpm.
Viable cell counts were determined by trypan blue dye exclusion.
Cells grown in suspension u937 hl 60 a6876 p3hr1 and adherent cells were transferred to 12 15 mm round bottomed centrifuge tubes.
